Fundamentals of Fluorescence Microscopy
By:Partha Pratim Mondal,Alberto Diaspro
Published on 2013-12-12 by Springer Science & Business Media
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
This Book was ranked at 14 by Google Books for keyword spectrum science exploring matter and energy 7 12 years.
Book ID of Fundamentals of Fluorescence Microscopy's Books is nc7HBAAAQBAJ, Book which was written byPartha Pratim Mondal,Alberto Diasprohave ETAG "9AYpTzrfXRo"
Book which was published by Springer Science & Business Media since 2013-12-12 have ISBNs, ISBN 13 Code is 9789400775459 and ISBN 10 Code is 9400775458
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